Ultimate Guide to Enokitake: Health Benefits & Uses

For centuries, Enokitake has been a cherished mushroom in East Asian traditions, both as a nourishing food and a natural remedy. Known for its slender stems and delicate flavor, it has long been a staple in comforting broths and wellness tonics, especially during colder seasons when immunity and strength were most valued.

More than just a culinary favourite, Enokitake was often regarded as a mushroom that supports vitality and longevity. Traditional healers believed it could help maintain balance in the body and protect against everyday ailments.

Now, modern science is beginning to catch up with this ancient wisdom. Researchers are taking a closer look at Enokitake’s unique compounds and uncovering its potential to support various aspects of health. What was once known through experience is now being validated through research.

In this guide, we’ll explore the evolving science behind Enokitake’s health potential, the various reported benefits and how to choose the right Enokitake supplements to support your modern wellness journey.

Index

• What are Functional Mushrooms?
  
• What Is Enokitake?
• The Science Behind The Benefits Of Enokitake
• Health Benefits of Enokitake
• How To Buy A Good Quality Enokitake Supplement?
• Dose, Safety, Side Effects
• How To Take Enokitake Mushrooms For Health Support
• Frequently Asked Questions
• Resources

What are Functional Mushrooms?

Referred to as 'functional mushrooms', these edible fungi boast a diverse range of bioactive compounds. Each type of functional mushroom possesses a unique bioactive profile, contributing to its ability to support specific bodily systems. Enokitake, in particular, stands out for its cardiovascular support, among other health benefits.

If you're familiar with functional mushrooms, you've likely encountered the term 'beta-glucans' or 'beta-D-glucans.' These polysaccharides play a crucial role in the immune-modulating effects of mushrooms. A more detailed exploration of Beta-Glucans will be provided in a dedicated section.

What Is Enokitake?

Enokitake is a highly sought-after edible mushroom and ranks as one of the four most commonly cultivated mushrooms worldwide, thanks to its appealing flavour, aroma, and impressive nutritional profile [1]. Enokitake is scientifically known as Flammulina velutipes and other common names of Enokitake include “velvet shank”, “golden needle mushroom” and “winter mushroom.”

Enokitake has been grown for both consumption and medicinal purposes in China since around 800 AD.

Cultivated Enokitake mushrooms have a white, bean sprout-like appearance with a velvety stem and small, snowy-white cap, while wild varieties range from orange to brown and feature larger, glossy caps. The difference in appearance is due to the absence of light exposure during cultivation, which results in the white colour, while wild mushrooms remain brown [1].

Enokitake typically grows on dead elm trees, especially those affected by Dutch elm disease [2], and can also be found on various deciduous trees like poplar, plum, maple, and birch [3]. This mushroom species is widespread in temperate regions of North America, Europe, and Asia [2].

The Science Behind The Benefits Of Enokitake

Employing modern scientific approaches, researchers have uncovered a wide array of potential health benefits linked to Enokitake. This mushroom contains a diverse range of bioactive compounds—including polysaccharides, immunomodulatory proteins, sterols, and dietary fibers—that work together to support immune function, cellular health, and overall well-being.

Polysaccharides

Recent studies have increasingly focused on the bioactive properties of polysaccharides extracted from both the fruiting body and mycelium of Enokitake. These polysaccharides have been shown to offer various health benefits, including antioxidant and anticancer effects, immune system modulation and potential support for learning and memory function [4, 5, 6, 7, 8]. Among these, beta-glucan is particularly notable for its demonstrated anticancer activity [1].

Beta-Glucans

Beta-glucans are naturally occurring polysaccharide compounds found in the cell walls of certain grains, fungi, bacteria, and other sources.

Beta-glucans function as Biological Response Modifiers (BRMs), modulating the immune system and adjusting its activity as needed. These modifiers have the capacity to enhance the immune response, leading to a more effective defence against infections and various other benefits [9].

Dietary Fiber

Most mushrooms contain high dietary fibre content, with dietary fibre making up approximately 29.3% of the dry weight of raw Enokitake mushrooms [10].

A previous study showed that the high fibre content in Enokitake extract helped reduce cholesterol levels in animals that were fed a high-fat diet, contributing to lower blood fat levels [11].

Flammulina velutipes polysaccharide I-A (FVP I-A)

Another noteworthy polysaccharide found in Enokitake is Flammulina velutipes polysaccharide I-A (FVP I-A), which has been shown to reduce oxidative stress and stimulate the immune system to provide an enhanced immune response to pathogens [6].

Immunomodulatory Proteins

Several bioactive proteins have been identified in Enokitake, each contributing to its potential functional properties:

• FIP-fve: A fungal immunomodulatory protein (FIP), primarily extracted from the fruiting body. It has been widely studied for its immune-regulating, anticancer, and anti-inflammatory properties [12, 13, 14].
• Ribosome-inactivating proteins (RIPs): These include flammin, velin, velutin, and flammulin, found in the fruiting body and extracts [15,16, 17]. Ribosome-inactivating proteins have several potential effects, such as stopping protein production in cells, breaking down certain molecules, blocking cell growth, and helping the immune system. RIPs have also been used in cancer treatments, where they are attached to toxins to target cancer cells [18].
• Hemagglutinin: This protein has been reported to exhibit mitogenic effects, meaning it can stimulate cell division, and antiproliferative effects, meaning it can prevent excessive cell growth [19].
• Ice-binding proteins and flammutoxin: Also present in Enokitake these compounds include flammutoxin, a known cytolysin that can disrupt cell membranes and potentially eliminate harmful cells [20, 21].

Lipids and fatty acids

• Linoleic acid: Linoleic acid is the main type of fat found in Enokitake mushrooms. It is an essential omega-6 fatty acid, meaning our bodies need it but cannot make it on their own—we must get it from food. This fatty acid helps create important substances in the body, such as prostaglandins (which regulate things like inflammation and blood flow) and arachidonic acid (which is involved in cell function and immune responses) [22].
• Functional sterols: Researchers have identified sterols in Enokitake mushrooms, specifically ergosterol, which has demonstrated antiproliferative effects—meaning they can slow down the growth of cancer cells in lab studies [23, 24].
• Mycosterol: Mycosterol, a compound found in Enokitake mushrooms, has been shown in animal studies to reduce blood lipid levels, suggesting it may have hypolipidemic effects—meaning it could help lower fat levels in the blood [11].

Terpenoids

Enokitake also contains sesquiterpenes and norsequiterpenes, which showed a range of bioactivities, including anticancer, antibacterial, and antioxidant effects [25, 26].

Polyketides

Lovastatin has been found to be present in the fruiting bodies of Enokitake [27]. Lovastatin is classified as a polyketide, which is a type of compound produced through a specific biosynthesis process. It is also a statin, meaning it inhibits an enzyme involved in cholesterol production, helping to reduce cholesterol levels in the body.

Other Noteworthy Compounds

Amino Acids

Enokitake contains both essential (e.g. methionine, valine, leucine) and non-essential amino acids (e.g. glutamic acid, alanine, glycine), with major contributors including glutamic acid, alanine, glycine, and lysine [28, 29, 30]. Together, these amino acids potentially contribute to overall metabolic health, immune function, muscle maintenance, and even neurological balance.

Amino acids also influence the mushroom’s mild and pleasant taste. Compounds like aspartic acid and glutamic acid create an umami (MSG-like) flavour, while alanine, glycine, and serine add sweetness [30].

One noteworthy amino acid to highlight is ergothioneine, a naturally occurring amino acid derivative often referred to as a “longevity vitamin” due to its powerful antioxidant properties [48-52].

Vitamins

Enokitake has been shown to contain:

• Tocopherols (Vitamin E) – Known for their potential antioxidant properties.
• Ascorbic acid (Vitamin C) – Supports skin, immune, and cellular health.
• Beta-carotene & lycopene – Powerful plant compounds often associated with healthy ageing and antioxidant activity [31, 32].

Minerals

Enokitake offers a spectrum of trace minerals that may support day-to-day health.
Potassium is the most dominant mineral in Enokitake, followed by phosphorus, making it a heart-friendly food thanks to its high potassium and low sodium content [1].

Health Benefits of Enokitake

Health Benefits at a Glance:

• Enokitake May Support Heart Health 
• Enokitake May Enhance Cognitive Function
• Enokitake May Help Calm Inflammation
• Enokitake May Strengthen the Immune System
• Enokitake May Help Fight Bacteria and Viruses
• Enokitake May Support Respiratory Health and Allergy Relief
• Enokitake May Enhance Skin Health
• Enokitake’s Anti-Tumour and Anti-Cancer Potential

Enokitake May Support Heart Health

Several studies have shown how Enokitake may contribute to heart health.

Enokitake is rich in natural compounds like dietary fiber and mycosterols—a type of plant sterol similar to cholesterol. These components have been shown to help maintain healthy cholesterol levels.

In a study using both Enokitake extract and Enokitake powder, animals on a high-fat diet experienced significant reductions in total cholesterol, LDL (“bad cholesterol”), triglycerides, and the LDL/HDL ratio when supplemented with Enokitake extract or powder [11]. These improvements are crucial for reducing risk factors linked to cardiovascular disease.

Another study found that Enokitake fiber not only lowered serum cholesterol, but also increased LDL receptor activity in the liver [33].

LDL receptors help your liver remove LDL (“bad”) cholesterol from the bloodstream. By enhancing these receptors, Enokitake supports the body’s natural ability to clear out excess cholesterol, keeping arteries healthier.

The same study also noted an increase in short-chain fatty acids (SCFAs) like butyric acid and acetic acid in the gut when rats were fed Enokitake fiber [33]. These SCFAs are known to support gut health and metabolic balance, which play an indirect but important role in cardiovascular health.

Enokitake May Enhance Cognitive Function

Enokitake mushrooms are gaining attention for their potential to enhance cognitive function, thanks to the unique polysaccharides they contain.

The brain relies on a chemical called acetylcholine to support memory and learning. Enokitake polysaccharides have been shown to increase acetylcholine levels by boosting the enzyme that produces it (choline acetyltransferase) and reducing the enzyme that breaks it down (acetylcholinesterase, also known as AChE) [7].

In other words, Enokitake helps maintain healthy levels of this essential neurotransmitter, which plays a central role in keeping your mind clear, focused, and sharp.

In lab tests with memory-impaired rats, Enokitake polysaccharides improved performance in a maze—specifically, the rats were able to find a hidden platform faster and more easily. This suggests enhanced spatial memory and learning ability [7].

To achieve this, Enokitake activated specific brain pathways linked to learning, including Connexin 36 and phosphorylated CaMK II, both of which are important for communication between brain cells.

Enokitake doesn’t just help with neurotransmitters—it also supports the brain’s natural defences. The mushroom’s polysaccharides were found to boost levels of powerful antioxidants like superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) [7].

These antioxidants help neutralise free radicals and reduce oxidative stress, which can otherwise damage brain cells and contribute to cognitive decline over time.

In a separate study, Enokitake polysaccharides demonstrated notable acetylcholinesterase(AChE)-inhibiting activity [34]. When AChE is inhibited, it prevents the breakdown of acetylcholine, allowing more of it to remain available in the brain for longer periods.

This can support improved cognitive functions like memory and focus, as more acetylcholine is available for communication between brain cells.

Enokitake May Help Calm Inflammation

Inflammation is the body’s natural response to injury or infection, but when it becomes chronic, it can contribute to everything from joint discomfort to age-related diseases. Enokitake mushrooms may offer gentle support in keeping this process in check.

In laboratory studies, Enokitake has shown a strong anti-inflammatory effect on immune cells. For example, when mouse immune cells (called macrophages) were stimulated to mimic inflammation, Enokitake was able to reduce the production of nitric oxide (NO), a molecule that, in excess, contributes to inflammation and cell damage [35, 36].

Even more impressively, Enokitake also suppressed key inflammatory proteins like iNOS and COX-2, which are involved in producing inflammatory chemicals in the body [36]. By dialing these down, Enokitake may help reduce the inflammatory response at its source.

The active compounds behind these effects are likely water-soluble antioxidants and polysaccharides found in Enokitake. These compounds also helped protect nerve-like cells from cell death when exposed to oxidative stress [36].

Enokitake May Strengthen the Immune System

One of Enokitake’s standout benefits is its potential ability to modulate the immune system—helping it respond effectively without overstimulation.

A standout protein in Enokitake is FIP-fve, a fungal immunomodulatory protein (FIP) shown to activate specific immune cells. This protein has been shown to work in the following ways:

• Enhances immune balance: FIP-fve helps steer the immune system toward Th1 dominance, which supports the body’s defense against viruses and harmful microbes, while also reducing IgE responses that are linked to allergies [37, 38]. This may help the body fight infections more effectively while also reducing allergy symptoms.
• Boosts T-cell activity: FIP-fve stimulates the growth of human lymphocytes (a type of white blood cell) and boosts production of IFN-γ, an important immune messenger, through the p38 MAPK pathway [38]. This can help keep the immune system more alert and responsive when the body needs it most.
• Improves immune memory: FIP-fve activates pathways like calcium signaling and PKC-α, which help immune cells produce IFN-γ more effectively [39]. This could lead to a stronger and more lasting immune response after the body encounters a pathogen.
• Reduces allergic responses: FIP-fve helps calm eosinophils, which are involved in allergic inflammation, by encouraging their natural death and lowering levels of IL-5 receptors, which are often elevated in allergies and asthma [40]. This may help reduce allergy flare-ups and support easier breathing in conditions like asthma.

Another key immune-supporting compound in Enokitake is its polysaccharides, which support the immune system in the following ways:

• Boosts immune signaling: A specific Enokitake polysaccharide, FVP I-A, was shown to increase important immune messengers like IL-1β, IL-6, and TNF-α, and stimulate the production of nitric oxide (NO)—a molecule that helps immune cells destroy harmful invaders [6]. This may help the immune system respond more quickly and effectively when faced with viruses, bacteria, or other pathogens.
• Promotes lymphocyte activity: FVP I-A increased the activation of both innate immune cells (which act fast) and adaptive immune cells (which target specific threats) [6]. This suggests a more balanced and well-rounded immune response, helping your body defend itself better across the board.

Enokitake May Help Fight Bacteria and Viruses

Enokitake doesn't just support immune balance—it may also play an active role in protecting the body against invading pathogens like viruses and harmful bacteria.

Enokitake contains several powerful proteins called ribosome-inactivating proteins (RIPs). These naturally occurring proteins interfere with the ability of viruses and bacteria to make new proteins—essentially shutting down their ability to replicate and spread. 

• Velutin, a low-molecular-weight RIP found in Enokitake, has been shown to inhibit HIV-1 reverse transcriptase—a key enzyme the virus uses to replicate. It also blocks enzymes like β-glucosidase and β-glucuronidase, which some bacteria use for survival and pathogenic activity [41]. 
• Other RIPs from Enokitake include flammin, velin, and flammulin—each with different molecular weights but similar activity. In lab experiments, these compounds stopped the process that cells use to build new proteins [42, 43]. While they don’t act like antibiotics, their ability to block essential viral or microbial processes suggests potential for infection support.

As explained in the earlier Enokitake May Strengthen the Immune System section of this guide, Enokitake offers more than direct inhibition of pathogens. It also works with your immune system to improve responsiveness:

• A specific polysaccharide extracted from the fruiting body—known as FVP I-A—was shown to stimulate the production of key immune messengers, including IL-1β, IL-6, and TNF-α, and to boost nitric oxide (NO) levels in immune cells [6]. NO plays a vital role in helping immune cells neutralize harmful bacteria and viruses more quickly. 
• Additionally, the FIP-fve protein from Enokitake helps shift the immune system toward Th1 dominance, which supports the body’s ability to fight viral and microbial threats while keeping allergy-type responses (Th2 dominance) in check [37, 38].

Enokitake May Support Respiratory Health and Allergy Relief

The FIP-fve protein found in Enokitake has also been explored for its benefits in respiratory health.
In several mouse studies, FIP-fve helped reduce inflammation in the airways, lowered allergic responses by reducing Th2 cytokines, and made it easier to breathe by reducing airway hyperreactivity. [14, 44]. This means Enokitake may help relieve asthma symptoms triggered by allergies.

Furthermore, FIP-fve reduced immune reactions caused by house dust mites, a common trigger for asthma [44]. FIP-fve also helped reduce the spread of respiratory syncytial virus (RSV) and inflammation in infected mice and cells [13].

Enokitake May Enhance Skin Health

Enokitake is gaining recognition in the world of natural skincare for its ability to support healthier, more radiant skin from the inside out. Thanks to its unique mix of antioxidants and immune-supporting polysaccharides, this mushroom may help protect skin, boost hydration, and slow visible signs of aging.

One of the key components of Enokitake is a polysaccharide called FVP I-A, which has been shown to neutralize harmful free radicals (unstable molecules from stress, sun, or pollution) like hydroxyl and superoxide anions [6]—two major contributors to premature aging and skin damage. This antioxidant activity helps protect skin cells from oxidative stress. Oxidative stress can lead to ongoing inflammation, damage important skin structures like collagen (which keeps your skin firm), and even interfere with how skin cells work. This can speed up skin aging and may contribute to other skin conditions [45].

Similarly, nucleotides from Enokitake have demonstrated strong antioxidant power in lab studies, with the ability to scavenge free radicals and maintain cellular health [46]. This may help skin stay clearer, smoother, and stronger.

In a randomized, double-blind, placebo-controlled study, participants who consumed a powdered form of Enokitake daily for four weeks experienced a significant increase in skin hydration compared to the placebo group. This suggests that compounds in Enokitake may enhance the skin’s natural moisture barrier, helping to retain hydration and reduce dryness [47].

This makes Enokitake a promising nutricosmetic—a type of supplement that works from the inside out to support healthy skin, hair, and nails [47].

Enokitake is rich in ergothioneine, a rare and powerful antioxidant found in high levels in this mushroom. Ergothioneine has been shown to protect against melanosis—the dark discoloration that can occur due to oxidation. Although this research was conducted in seafood models, the same antioxidant mechanism is relevant to human skin, where oxidative stress contributes to pigmentation issues and a loss of skin tone [48-52].

In fact, ESH appears to work by inhibiting polyphenoloxidase (PPO) and prophenoloxidase (proPO), enzymes that drive oxidative pigmentation changes. It also demonstrates copper-chelating activity, which further reduces oxidative stress—a major factor in uneven skin tone and premature aging [51].

The FVP I-A polysaccharide also stimulates the immune system in ways that benefit the skin. It increases the production of nitric oxide and key cytokines like IL-1β and IL-6, which can enhance the skin’s natural defenses and healing capacity [6]. When skin is exposed to pollutants or UV rays, a strong immune response helps it recover faster and more effectively.

Enokitake’s Anti-Tumour and Anti-Cancer Potential

Enokitake’s active ingredients are being explored for their potential to inhibit tumor development, slow cancer cell proliferation, and even support those undergoing chemotherapy. The most notable compounds responsible for Enokitake’s anti-cancer benefits are sterols, FIP-fve and polysaccharides. The research on Enokitake and its effect on various cancers will be discussed in depth below.

General Anti-Cancer Potential Benefits

Enokitake’s fruiting bodies are rich in ergosterol [53]—a naturally occurring compound with promising anti-cancer potential. 

In laboratory studies, sterols extracted from Enokitake mushrooms were found to slow down the growth of liver and lung cancer cells. What’s especially promising is that these effects were seen even at very low doses, suggesting that only a small amount was needed to have a noticeable impact. [54]. To overcome their poor solubility, these sterols were encapsulated in liposomes or nanomicelles, which greatly improved their absorption and delivery to key organs like the liver and spleen—potentially enhancing their effectiveness [54, 55].

Another compound has been found to “train” immune cells to recognise and fight tumours. An earlier mentioned protein called FIP-fve also plays a role in helping the immune system recognize and respond to tumors more effectively. This protein was shown to boost both the innate and adaptive immune responses in mice, significantly increasing the release of interferon-gamma (IFN-γ)—a key signal that helps immune cells attack cancer cells. FIP-fve also enhanced the expression of molecules that help immune cells identify abnormal cells, suggesting it could support the body’s natural ability to detect and target tumors [56].

Polysaccharides from Enokitake mushrooms have been shown to slow down the growth of liver cancer cells in lab studies. They do this by reducing how often the cells divide, lowering their energy production, and stopping them from spreading. In animal studies, these effects also led to smaller tumor sizes, suggesting these mushroom compounds may help weaken cancer cells and slow their growth [57].

Enokitake and Liver Cancer

Sterols from the fruiting body of Enokitake, like ergosterol and 22,23-dihydroergosterol, have been shown to help stop the growth of liver cancer cells in lab tests. When these sterols were delivered in special forms (liposomes or nanomicelles), they were more effectively absorbed and targeted to the liver, making them more useful for liver support [54, 55]. 

Additionally, a different study found that FIP-fve (immunomodulatory protein FVE), helped extend the lifespan of mice with liver tumors, shrink the tumor, and boost the activity of immune cells [56]. It also stopped new blood vessels from forming in the tumor, which can slow down the cancer from spreading.

Enokitake and Brain Cancer

One study found that sterols from Enokitake significantly suppressed the growth of glioblastoma cells (U251), with better results than 5-fluorouracil, a common chemo drug. The effect was enhanced further when the sterols were delivered in nano-sized micelles, boosting absorption and targeting [55].

Enokitake and Lung Cancer

FIP-fve, a protein found in Enokitake mushrooms, helped stop lung cancer cells (A549) from growing by turning on a protective protein called p53 and its partner p21. It also prevented the cancer cells from spreading by blocking a key protein, RACGAP1, and disrupting the Rac1 pathway, which helps cancer cells move around [58].

Enokitake’s proteins also appear to reduce some of the damage caused by chemotherapy (docetaxel, which is widely used in the treatment of non-small cell lung cancer), protecting bone marrow, small intestines, and preserving bone structure in treated mice. FIP-fve helped reduce inflammation markers and supported better recovery without interfering with treatment [59].

Enokitake and Cervical Cancer

Enokitake sterols, especially those containing ergosterol and 22,23-dihydroergosterol, were found to reduce the survival of HeLa cells, which are cervical cancer cells. The more sterols were used, the greater the decrease in cancer cell survival.

However, HeLa cells were less affected compared to other cancer cells, like U251 (a brain cancer model), which showed a stronger response to the same treatment [55]. This suggests that while Enokitake sterols can help fight cervical cancer, their effectiveness may differ depending on the type of cancer.

Enokitake and Breast Cancer

In one study, Enokitake showed strong effects on breast cancer cells, both hormone receptor-positive and negative. It stopped the growth of these cells, caused them to die quickly, and almost completely prevented the cancer cells from forming new colonies in lab tests [60].

How To Buy A Good Quality Enokitake Supplement?

Choosing a good quality mushroom supplement can be a daunting task, as there are many options available in the market. However, there are a few key things to consider when selecting a high-quality mushroom supplement:

Fruiting Body vs. Mycelium

Quality control standards for functional mushroom products are currently lacking, highlighting the urgent need for systematic scientific verification of active compounds in these nutritional supplements. This is crucial for ensuring greater accountability regarding ingredient integrity and transparency.

When a product fails to specify the source of its Enokitake, there's a risk that it relies on mycelium (the fungal root structure) rather than the Enokitake fruiting body (the actual mushroom we see above the ground). To ensure the highest quality Enokitake mushroom supplement, it is advisable to choose products with an ingredients label indicating the use of only organic fruiting body, free from mycelium or grain contaminants.

Explore the notable distinctions between supplements crafted from mycelium and those derived from the fruiting body by clicking here.

Beta-Glucan Content

Beta-glucans are one of the primary compounds responsible for the various health benefits associated with mushrooms.

Many brands incorporate various starch, fillers, colourings, unnecessary additives, and other ingredients into their supplements, which offer no health benefits. Moreover, these additions suggest a low percentage of actual Enokitake extract in the product, resulting in a diminished concentration of beta-glucans (medicinal compounds) and, consequently, lower therapeutic potency.

To obtain the most potent and high-value functional fungi supplement, scrutinise the product label for specific beta-glucan content. To ensure the optimal quality of your Enokitake mushroom supplement, verify that the product specifies a beta-glucan concentration of at least 25%. This ensures you receive the full spectrum of health benefits associated with this mushroom.

Antioxi's Enokitake Extract boasts a beta-glucan content exceeding 30%, guaranteeing that you receive the necessary benefits.

Organically Certified

Opt for certified organic options as mushrooms tend to absorb pollutants from their surroundings. Mushrooms that are organically grown refer to mushrooms cultivated in a natural manner, devoid of herbicides, fungicides, or pesticides.

Safety Tests

Heavy metals and pesticides tests are safety tests which will indicate whether mushrooms are safe to consume.

At Antioxi, all our mushrooms are third-party tested and verified to ensure heavy metals, mycotoxins, pesticides, herbicides, microbial contaminants, ethylene oxide (ETO), and polycyclic aromatic hydrocarbons (PAHs) are all within safe limits.

Extraction Method

Understanding the ingredients in your diet is crucial, especially when it comes to functional mushrooms and their quality. Opting for a low-quality mushroom supplement poses a risk to experiencing the full range of potential benefits, making your health a priority.

A key indicator of a high-quality functional mushroom product is its extraction from the mushroom (fruiting body) and using the appropriate method. Three commonly used extraction methods for functional mushrooms are hot water, alcohol, and dual extraction (involving both hot water and alcohol).

The choice of the extraction method can impact the quality, and research indicates that not all methods are equally effective for every mushroom. Some methods may even remove medicinal constituents during the process. Therefore, it is crucial for supplement producers to understand which extraction method ensures maximum benefit and efficacy for their product.

• Hot water extraction involves heating the mushroom in water to draw out water-soluble compounds like polysaccharides and beta-glucans. The resulting liquid can be consumed directly or dried to create a powder concentrated with the beneficial compounds.
• Alcohol extraction follows a comparable procedure but utilises alcohol instead of water to eliminate non-water-soluble compounds such as triterpenes.

Determining the optimal extraction method, whether hot water or alcohol, depends on the mushroom in question. Antioxi employs a hot water extraction approach for Enokitake.

Through the extraction processes, the liquids obtained are then evaporated, resulting in a single extract powder. This method ensures the highest potency and health benefits in the final product.

To guarantee the top-notch quality of your Enokitake mushroom supplement, check whether the product has utilised a hot water extraction method.

Click the link below to learn more about what to look for when choosing a high-quality mushroom supplement.

Learn More

Read the Supplement Label

To make an informed decision, it’s essential to understand how to read supplement labels correctly. Labels can often be misleading, and knowing what to look for, such as beta-glucan content, fruiting body vs. mycelium, and extraction method, can help you avoid low-quality products.

Please read our full guide on how to decode supplement labels here: How to Read Supplement Labels

Click the link above to learn more about what to look for when choosing a high-quality mushroom supplement.

Dose, Safety, Side Effects

Dose

Baseline Dose
Start with 2 capsules or 1 gram per day for general wellness. This helps you experience the foundational benefits of Enokitake.

Enhanced Dose
For more pronounced effects, please get in touch with us. Our team can help you determine the best approach for your needs.

Flexible Dosage Regimen
Whether you prefer splitting the dose throughout the day or taking it all at once, the choice is yours. For optimal absorption, it is recommended to consume mushrooms on an empty stomach. However, if you have a sensitive constitution, consider splitting the dose and taking it after a meal.

Feeling unsure about where to begin? Schedule your free private online consultation with Marko, our Founder, and discover the perfect products to meet your wellness goals. Click here to book a free consultation with Marko.

Safety

Individuals with diabetes are advised to seek guidance from their healthcare provider before incorporating Enokitake into their diet, as it may impact blood sugar levels.

While some studies suggest that Enokitake may have anti-inflammatory effects, it can also stimulate the immune system. Therefore, individuals with autoimmune diseases should seek medical guidance before considering the use of Enokitake.

Do not consume Enokitake mushrooms within two weeks of undergoing surgery or if you suffer from a bleeding disorder.

For individuals who are pregnant, breastfeeding, or have an autoimmune disease, it is crucial to consult with a healthcare professional before considering the use of Enokitake mushrooms.

Do not consume Enokitake if you are allergic to mushrooms.

MEDICATION INTERACTIONS

While Enokitake mushrooms boast potential health benefits, it's crucial to be mindful of potential interactions with medications. Individuals who are taking the following medications should also consult their healthcare provider before incorporating Enokitake into their regimen:

• Medications for diabetes management
• Blood-thinning medications
• Medications to suppress the immune system

If you have any concerns regarding the interaction between Enokitake and your medications, it's a good idea to discuss it with your healthcare provider. They can offer you the most appropriate guidance.

Please bear in mind that the information we provide is for educational purposes and shouldn't be considered a replacement for professional medical advice.

Your health and safety are important to us and we want to ensure all our customers use our products to their benefit, not detriment.

Side Effects

A recent clinical trial involving healthy adults found that taking dried Enokitake powder daily for four weeks not only improved skin hydration but also caused no reported side effects among participants [47].

Additionally, animal studies have shown that even high oral doses of Enokitake did not cause visible toxicity or tissue damage in mice [61].

One particular compound found in Enokitake, flammutoxin, is known to be harmful when injected into the body. It can affect red blood cells and heart function—but here's the good news: flammutoxin is not considered toxic when eaten, and it’s also deactivated by heat during cooking [61].

Although rare, there have been isolated reports of allergic reactions, including anaphylaxis, after consuming Enokitake. In two separate Japanese case studies, individuals with no prior mushroom allergies developed symptoms such as oral itching and full-body allergic responses after eating dishes that included Enokitake [62, 63].

In an animal study, mice were fed large amounts of dried Enokitake daily for five consecutive days—doses equivalent to 190–280 grams of fresh mushrooms per day for an average adult. At the highest doses, researchers observed mild signs that muscles and heart tissue may be slightly affected, such as increases in certain enzymes linked to muscle strain (e.g. creatine kinase and CK-MB). However, no actual tissue damage or inflammation was found under the microscope [61].

How To Take Enokitake Mushrooms For Health Support

Powders vs. Capsules

For those with a fast-paced lifestyle, intricate recipes might not be in the cards. That's precisely why Antioxi has crafted an Organic Enokitake Extract that is available in convenient capsule form.

If you're a cooking enthusiast or favour the convenience of a powder, our Organic Enokitake extract in powder form could be an ideal option for you. Our Enokitake can be seamlessly incorporated into smoothies, stews, coffee and all your other favourite meals and drinks.

It's essential to note that there is no difference in potency between our powdered extracts and capsules. Our capsules contain the exact same powdered extract, guaranteeing uniform effectiveness throughout our product line.

Frequently Asked Questions

Are The Mushrooms Organic?

Our Agaricus Blazei is organically sourced and certified.

Is It Safe To Consume Medicinal Mushrooms During Pregnancy Or Whilst Breastfeeding?

While medicinal mushrooms can offer some great benefits during pregnancy such as strengthening immune health, improving digestion and of course the much needed energy boost, there is unfortunately not yet enough information regarding studies during pregnancy and whilst breastfeeding where we can confidently give advice.

The best would be to consult with your healthcare provider and/or midwife.

Can Children Use Medicinal Mushrooms?

Research regarding the use of medicinal mushrooms by children is still at its infancy. There is however an interesting study conducted in 2018 which investigated the effects of Reishi on immune system cells of 3-5 year olds. [64]

The study showed that Reishi increased immune system cell counts in the peripheral blood, which are crucial for defending against infections. The treatments were also well-tolerated and safe, with no abnormal increases in serum creatinine or hepatic aminotransferases. While the study shows promise in the safety and effectiveness of the use of medicinal mushrooms in children, we do always suggest consulting with your child's doctor prior to introducing anything new into their diet.

If you do get the go ahead we suggest reducing the diet to 1/4 of a dose for young children.

These findings suggest the need for more extended controlled clinical trials to evaluate the effectiveness of medicinal mushrooms in preventing infections in children.

What Is The Difference Between The Powder And Capsules?

There is no difference in terms of benefits. The only difference is preference of use.

What Is The Difference Between Using The 8 Mushroom Blend And Using An Individual Mushroom?

Our 8-blend mushroom product is like an all-in-one health elixir. It's perfect for those seeking overall well-being, boosting digestion, or just looking for a daily health lift.

However, if you're using mushrooms as targeted support for a specific health concern, say, Lion's Mane for cognitive clarity or Reishi for stress relief, the individual route is your best bet.

Are There Any Allergy Precautions/Medication Interactions?

Individuals with diabetes are advised to seek guidance from their healthcare provider before incorporating Enokitake into their diet, as it may impact blood sugar levels.

While some studies suggest that Enokitake may have anti-inflammatory effects, it can also stimulate the immune system. Therefore, individuals with autoimmune diseases should seek medical guidance before considering the use of Enokitake.

Do not consume Enokitake mushrooms within two weeks of undergoing surgery or if you suffer from a bleeding disorder.

For individuals who are pregnant, breastfeeding, or have an autoimmune disease, it is crucial to consult with a healthcare professional before considering the use of Enokitake mushrooms.

Do not consume Enokitake if you are allergic to mushrooms.

While Enokitake mushrooms boast potential health benefits, it's crucial to be mindful of potential interactions with medications.

Individuals who are taking the following medications should also consult their healthcare provider before incorporating Enokitake into their regimen:

• Medications for diabetes management
• Blood-thinning medications
• Medications to suppress the immune system

If you have any concerns regarding the interaction between Enokitake and your medications, it's a good idea to discuss it with your healthcare provider. They can offer you the most appropriate guidance.

Please bear in mind that the information we provide is for educational purposes and shouldn't be considered a replacement for professional medical advice. 

Which Mushroom Extraction Do You Use?

The optimal extraction method once again depends on the mushroom itself. Different methods are used to promote and dissolve the unique active ingredients in the mushroom extracts and hence create a high spectrum mushroom extract.

The 3 different extraction methods:

1. Alcohol (ethanol)

This extraction is used to draw out the fat-soluble compounds in the mushrooms, such as the adaptogenic terpenoids.

2. Hot water

This extraction is used to extract the water-soluble compounds, the polysaccharides, and the immunomodulating Beta-D-glucans. The dual extraction process ensures you’re getting the full spectrum of compounds when it comes to a potent mushroom extract.

3. Dual extraction

Dual extraction refers to a combination of water and alcohol extraction. The dual extraction process ensures you’re getting the full spectrum of compounds when it comes to a mushroom where the active ingredients benefit from both alcohol and well as a water extract.

Hot water extraction works best for:

Cordyceps, Turkey Tail, Maitake, Tremella, Shiitake, Enokitake

Double (alcohol & water) extraction works best for:

Lion's Mane, Reishi, Chaga

Do You Use The Fruiting Body Or Mycelium?

We use the Fruiting Body of Enokitake.

We have a helpful article here which explains the different mushroom parts.

Resources

1. Tang, C., Hoo, P. C., Tan, L. T., Pusparajah, P., Khan, T. M., Lee, L. H., Goh, B. H., & Chan, K. G. (2016). Golden Needle Mushroom: A Culinary Medicine with Evidenced-Based Biological Activities and Health Promoting Properties. Frontiers in pharmacology, 7, 474. https://doi.org/10.3389/fphar.2016.00474
2. Ingold, C. T. (1980). Flammulina velutipes. Bulletin of the British Mycological Society, 14(2), 112-118. https://doi.org/10.1016/S0007-1528(80)80006-X
3. Sharma, V., Kumar, S., & Tewari, R. (2009). Flammulina velutipes, the culinary medicinal winter mushroom. Directorate of Mushroom Research, Indian Council of Agricultural Research.
4. Pang, X., Yao, W., Yang, X., Xie, C., Liu, D., Zhang, J., & Gao, X. (2007). Purification, characterization and biological activity on hepatocytes of a polysaccharide from Flammulina velutipes mycelium. Carbohydrate Polymers, 70(3), 291–297. https://doi.org/10.1016/j.carbpol.2007.04.010
5. He, W., & Zhang, Z. (2013). Antioxidant activities of polysaccharides of Flammulina velutipes obtained by ultrafiltration. AgroFOOD Industry Hi Tech, 24(4), 53–56. https://www.teknoscienze.com/tks_article/antioxidant-activitiesof-polysaccharides-of-flammulina-velutipes-obtained-by-ultra-filtration/
6. Wu, M., Luo, X., Xu, X., Wei, W., Yu, M., Jiang, N., Ye, L., Yang, Z., & Fei, X. (2014). Antioxidant and immunomodulatory activities of a polysaccharide from Flammulina velutipes. Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 34(6), 733–740. https://doi.org/10.1016/s0254-6272(15)30089-3
7. Yang, W., Yu, J., Zhao, L., Ma, N., Fang, Y., Pei, F., Mariga, A. M., & Hu, Q. (2015). Polysaccharides from Flammulina velutipes improve scopolamine-induced impairment of learning and memory in rats. Journal of Functional Foods, 18(Part A), 411–422. https://doi.org/10.1016/j.jff.2015.08.003
8. Zhang, Z., Jin, Q., Lv, G., Fan, L., Pan, H., & Fan, L. (2013). Comparative study on antioxidant activity of four varieties of Flammulina velutipes with different colour. International Journal of Food Science and Technology, 48(5), 1057–1064. https://doi.org/10.1111/ijfs.12062
9. Zhong, X., Wang, G., Li, F., Fang, S., Zhou, S., Ishiwata, A., Tonevitsky, A. G., Shkurnikov, M., Cai, H., & Ding, F. (2023). Immunomodulatory Effect and Biological Significance of β-Glucans. Pharmaceutics, 15(6), 1615. https://doi.org/10.3390/pharmaceutics15061615
10. Dikeman, C. L., Bauer, L. L., Flickinger, E. A., & Fahey, G. C., Jr (2005). Effects of stage of maturity and cooking on the chemical composition of select mushroom varieties. Journal of agricultural and food chemistry, 53(4), 1130–1138. https://doi.org/10.1021/jf048541l
11. Yeh, M. Y., Ko, W. C., & Lin, L. Y. (2014). Hypolipidemic and antioxidant activity of enoki mushrooms (Flammulina velutipes). BioMed research international, 2014, 352385. https://doi.org/10.1155/2014/352385
12. Chang, Y. C., Hsiao, Y. M., Wu, M. F., Ou, C. C., Lin, Y. W., Lue, K. H., & Ko, J. L. (2013). Interruption of lung cancer cell migration and proliferation by fungal immunomodulatory protein FIP-fve from Flammulina velutipes. Journal of agricultural and food chemistry, 61(49), 12044–12052. https://doi.org/10.1021/jf4030272
13. Chang, Y. C., Chow, Y. H., Sun, H. L., Liu, Y. F., Lee, Y. T., Lue, K. H., & Ko, J. L. (2014). Alleviation of respiratory syncytial virus replication and inflammation by fungal immunomodulatory protein FIP-fve from Flammulina velutipes. Antiviral research, 110, 124–131. https://doi.org/10.1016/j.antiviral.2014.08.006
14. Lee, Y. T., Lee, S. S., Sun, H. L., Lu, K. H., Ku, M. S., Sheu, J. N., Ko, J. L., & Lue, K. H. (2013). Effect of the fungal immunomodulatory protein FIP-fve on airway inflammation and cytokine production in mouse asthma model. Cytokine, 61(1), 237–244. https://doi.org/10.1016/j.cyto.2012.09.024
15. Wang, H. X., & Ng, T. B. (2000). Flammulin: a novel ribosome-inactivating protein from fruiting bodies of the winter mushroom Flammulina velutipes. Biochemistry and cell biology = Biochimie et biologie cellulaire, 78(6), 699–702. https://doi.org/10.1139/o00-087
16. Wang, H., & Ng, T. B. (2001). Isolation and characterization of velutin, a novel low-molecular-weight ribosome-inactivating protein from winter mushroom (Flammulina velutipes) fruiting bodies. Life sciences, 68(18), 2151–2158. https://doi.org/10.1016/s0024-3205(01)01023-2
17. Ng, T. B., & Wang, H. X. (2004). Flammin and velin: new ribosome inactivating polypeptides from the mushroom Flammulina velutipes. Peptides, 25(6), 929–933. https://doi.org/10.1016/j.peptides.2004.03.007 
18. Ng, T. B. (2006). Fungal ribosome inactivating proteins. Handbook of Biologically Active Peptides, 145–149. https://doi.org/10.1016/B978-012369442-3/50025-8
19. Ng, T. B., Ngai, P. H., & Xia, L. (2006). An agglutinin with mitogenic and antiproliferative activities from the mushroom Flammulina velutipes. Mycologia, 98(2), 167–171. https://doi.org/10.3852/mycologia.98.2.167
20. Raymond, J. A., & Janech, M. G. (2009). Ice-binding proteins from enoki and shiitake mushrooms. Cryobiology, 58(2), 151–156. https://doi.org/10.1016/j.cryobiol.2008.11.009
21. Tadjibaeva, G., Sabirov, R., & Tomita, T. (2000). Flammutoxin, a cytolysin from the edible mushroom Flammulina velutipes, forms two different types of voltage-gated channels in lipid bilayer membranes. Biochimica et biophysica acta, 1467(2), 431–443. https://doi.org/10.1016/s0005-2736(00)00240-6
22. Salem, N., Jr, Pawlosky, R., Wegher, B., & Hibbeln, J. (1999). In vivo conversion of linoleic acid to arachidonic acid in human adults. Prostaglandins, leukotrienes, and essential fatty acids, 60(5-6), 407–410. https://doi.org/10.1016/s0952-3278(99)80021-0 
23. Yi, C., Zhong, H., Tong, S., Cao, X., Firempong, C. K., Liu, H., Fu, M., Yang, Y., Feng, Y., Zhang, H., Xu, X., & Yu, J. (2012). Enhanced oral bioavailability of a sterol-loaded microemulsion formulation of Flammulina velutipes, a potential antitumor drug. International journal of nanomedicine, 7, 5067–5078. https://doi.org/10.2147/IJN.S34612
24. Yi, C., Fu, M., Cao, X., Tong, S., Zheng, Q., Firempong, C. K., Jiang, X., Xu, X., & Yu, J. (2013). Enhanced oral bioavailability and tissue distribution of a new potential anticancer agent, Flammulina velutipes sterols, through liposomal encapsulation. Journal of agricultural and food chemistry, 61(25), 5961–5971. https://doi.org/10.1021/jf3055278
25. Wang, H., Khor, T. O., Shu, L., Su, Z. Y., Fuentes, F., Lee, J. H., & Kong, A. N. (2012). Plants vs. cancer: a review on natural phytochemicals in preventing and treating cancers and their druggability. Anti-cancer agents in medicinal chemistry, 12(10), 1281–1305. https://doi.org/10.2174/187152012803833026 
26. Wang, Y., Bao, L., Yang, X., Li, L., Li, S., Gao, H., Yao, X. S., Wen, H., & Liu, H. W. (2012). Bioactive sesquiterpenoids from the solid culture of the edible mushroom Flammulina velutipes growing on cooked rice. Food chemistry, 132(3), 1346–1353. https://doi.org/10.1016/j.foodchem.2011.11.117
27. Chen, S.-Y., Ho, K.-J., Hsieh, Y.-J., Wang, L.-T., & Mau, J.-L. (2012). Contents of lovastatin, γ-aminobutyric acid and ergothioneine in mushroom fruiting bodies and mycelia. LWT - Food Science and Technology, 47(2), 274–278. https://doi.org/10.1016/j.lwt.2012.01.019
28. Smiderle, F. R., Carbonero, E. R., Sassaki, G. L., Gorin, P. A. J., & Iacomini, M. (2008). Characterization of a heterogalactan: Some nutritional values of the edible mushroom Flammulina velutipes. Food Chemistry, 108(1), 329–333. https://doi.org/10.1016/j.foodchem.2007.10.029
29. Kim, M.-Y., Chung, I.-M., Lee, S.-J., Ahn, J.-K., Kim, E.-H., Kim, M.-J., Kim, S.-L., Moon, H.-I., Ro, H.-M., Kang, E.-Y., Seo, S.-H., & Song, H.-K. (2009). Comparison of free amino acid, carbohydrates concentrations in Korean edible and medicinal mushrooms. Food Chemistry, 113(2), 386–393. https://doi.org/10.1016/j.foodchem.2008.07.045
30. Beluhan, S., & Ranogajec, A. (2011). Chemical composition and non-volatile components of Croatian wild edible mushrooms. Food Chemistry, 124(3), 1076–1082. https://doi.org/10.1016/j.foodchem.2010.07.081
31. Breene W. M. (1990). Nutritional and Medicinal Value of Specialty Mushrooms. Journal of food protection, 53(10), 883–894. https://doi.org/10.4315/0362-028X-53.10.883
32. Pereira, E., Barros, L., Martins, A., & Ferreira, I. C. F. R. (2012). Towards chemical and nutritional inventory of Portuguese wild edible mushrooms in different habitats. Food Chemistry, 130(2), 394–403. https://doi.org/10.1016/j.foodchem.2011.07.057
33. Fukushima, M., Ohashi, T., Fujiwara, Y., Sonoyama, K., & Nakano, M. (2001). Cholesterol-lowering effects of maitake (Grifola frondosa) fiber, shiitake (Lentinus edodes) fiber, and enokitake (Flammulina velutipes) fiber in rats. Experimental biology and medicine (Maywood, N.J.), 226(8), 758–765. https://doi.org/10.1177/153537020222600808
34. Yang, W., Fang, Y., Liang, J., & Hu, Q. (2011). Optimization of ultrasonic extraction of Flammulina velutipes polysaccharides and evaluation of its acetylcholinesterase inhibitory activity. Food Research International, 44(5), 1269–1275. https://doi.org/10.1016/j.foodres.2010.11.027
35. Gunawardena, D., Bennett, L., Shanmugam, K., King, K., Williams, R., Zabaras, D., Head, R., Ooi, L., Gyengesi, E., & Münch, G. (2014). Anti-inflammatory effects of five commercially available mushroom species determined in lipopolysaccharide and interferon-γ activated murine macrophages. Food chemistry, 148, 92–96. https://doi.org/10.1016/j.foodchem.2013.10.015 
36. Kang, H. W. (2012). Antioxidant and anti-inflammatory effect of extracts from Flammulina velutipes (Curtis) Singer. Journal of the Korean Society of Food Science and Nutrition, 41(8), 1072–1078. https://www.dbpia.co.kr/journal/articleDetail?nodeId=NODE01930280&language=ko_KR&hasTopBanner=true 
37. Hsieh, K. Y., Hsu, C. I., Lin, J. Y., Tsai, C. C., & Lin, R. H. (2003). Oral administration of an edible-mushroom-derived protein inhibits the development of food-allergic reactions in mice. Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 33(11), 1595–1602. https://doi.org/10.1046/j.1365-2222.2003.01790.x
38. Wang, P. H., Hsu, C. I., Tang, S. C., Huang, Y. L., Lin, J. Y., & Ko, J. L. (2004). Fungal immunomodulatory protein from Flammulina velutipes induces interferon-gamma production through p38 mitogen-activated protein kinase signaling pathway. Journal of agricultural and food chemistry, 52(9), 2721–2725. https://doi.org/10.1021/jf034556s
39. Ou, C. C., Hsiao, Y. M., Wu, W. J., Tasy, G. J., Ko, J. L., & Lin, M. Y. (2009). FIP-fve stimulates interferon-gamma production via modulation of calcium release and PKC-alpha activation. Journal of agricultural and food chemistry, 57(22), 11008–11013. https://doi.org/10.1021/jf902725s
40. Hsieh, C. W., Lan, J. L., Meng, Q., Cheng, Y. W., Huang, H. M., & Tsai, J. J. (2007). Eosinophil apoptosis induced by fungal immunomodulatory peptide-fve via reducing IL-5alpha receptor. Journal of the Formosan Medical Association = Taiwan yi zhi, 106(1), 36–43. https://doi.org/10.1016/S0929-6646(09)60214-X 
41. Wang, H., & Ng, T. B. (2001). Isolation and characterization of velutin, a novel low-molecular-weight ribosome-inactivating protein from winter mushroom (Flammulina velutipes) fruiting bodies. Life sciences, 68(18), 2151–2158. https://doi.org/10.1016/s0024-3205(01)01023-2
42. Ng, T. B., & Wang, H. X. (2004). Flammin and velin: new ribosome inactivating polypeptides from the mushroom Flammulina velutipes. Peptides, 25(6), 929–933. https://doi.org/10.1016/j.peptides.2004.03.007 
43. Wang, H. X., & Ng, T. B. (2000). Flammulin: a novel ribosome-inactivating protein from fruiting bodies of the winter mushroom Flammulina velutipes. Biochemistry and cell biology = Biochimie et biologie cellulaire, 78(6), 699–702. https://doi.org/10.1139/o00-087
44. Chu, P. Y., Sun, H. L., Ko, J. L., Ku, M. S., Lin, L. J., Lee, Y. T., Liao, P. F., Pan, H. H., Lu, H. L., & Lue, K. H. (2017). Oral fungal immunomodulatory protein-Flammulina velutipes has influence on pulmonary inflammatory process and potential treatment for allergic airway disease: A mouse model. Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi, 50(3), 297–306. https://doi.org/10.1016/j.jmii.2015.07.013 
45. Kruk, J., & Duchnik, E. (2014). Oxidative stress and skin diseases: possible role of physical activity. Asian Pacific journal of cancer prevention : APJCP, 15(2), 561–568. https://doi.org/10.7314/apjcp.2014.15.2.561 
46. Cheng, C.-L., Wang, Z.-Y., Cheng, L., Zhao, H.-T., Yang, X., Liu, J.-R., Cui, M.-L., & Wang, R.-C. (2012). In vitro antioxidant activities of water-soluble nucleotide-extract from edible fungi. Food Science and Technology Research, 18(3), 405–412. https://doi.org/10.3136/fstr.18.405
47. Nagae, M., Isa, A., Ishikawa, S., Muta, S., & Shimizu, K. (2023). Effect of Oral Intake of Flammulina velutipes (Enokitake) on Skin Condition in Healthy Adult Women: A Randomized, Double-Blind, Placebo-Controlled Study on Mental and Physical Health. Cosmetics, 10(2), 57. https://doi.org/10.3390/cosmetics10020057  
48. Encarnacion, A. B., Fagutao, F., Hirono, I., Ushio, H., & Ohshima, T. (2010). Effects of ergothioneine from mushrooms (Flammulina velutipes) on melanosis and lipid oxidation of kuruma shrimp (Marsupenaeus japonicus). Journal of agricultural and food chemistry, 58(4), 2577–2585. https://doi.org/10.1021/jf903944y
49. Encarnacion, A. B., Fagutao, F., Hirayama, J., Terayama, M., Hirono, I., & Ohshima, T. (2011). Edible mushroom (Flammulina velutipes) extract inhibits melanosis in Kuruma shrimp (Marsupenaeus japonicus). Journal of food science, 76(1), C52–C58. https://doi.org/10.1111/j.1750-3841.2010.01890.x 
50. Encarnacion, A. B., Fagutao, F., Shozen, K.-i., Hirono, I., & Ohshima, T. (2011). Biochemical intervention of ergothioneine-rich edible mushroom (Flammulina velutipes) extract inhibits melanosis in crab (Chionoecetes japonicus). Food Chemistry, 127(4), 1594–1599. https://doi.org/10.1016/j.foodchem.2011.02.023
51. Encarnacion, A. B., Fagutao, F., Jintasataporn, O., Worawattanamateekul, W., Hirono, I., & Ohshima, T. (2012). Application of ergothioneine-rich extract from an edible mushroom Flammulina velutipes for melanosis prevention in shrimp, Penaeus monodon and Litopenaeus vannamei. Food Research International, 45(1), 232–237. https://doi.org/10.1016/j.foodres.2011.10.030
52. Bao, H. N., Osako, K., & Ohshima, T. (2010). Value-added use of mushroom ergothioneine as a colour stabilizer in processed fish meats. Journal of the science of food and agriculture, 90(10), 1634–1641. https://doi.org/10.1002/jsfa.3992 
53. Wang, R., Ma, P., Li, C., Wang, Y., Wu, F., Jin, Y., Zhang, Y., Zhao, R., Gao, J., & Zhang, C. (2019). Combining transcriptomics and metabolomics to reveal the underlying molecular mechanism of ergosterol biosynthesis during the fruiting process of Flammulina velutipes. BMC Genomics, 20, 999. https://doi.org/10.1186/s12864-019-6370-1
54. Yi, C., Sun, C., Tong, S., Cao, X., Feng, Y., Firempong, C. K., Jiang, X., Xu, X., & Yu, J. (2013). Cytotoxic effect of novel Flammulina velutipes sterols and its oral bioavailability via mixed micellar nanoformulation. International journal of pharmaceutics, 448(1), 44–50. https://doi.org/10.1016/j.ijpharm.2013.03.020
55. Yi, C., Fu, M., Cao, X., Tong, S., Zheng, Q., Firempong, C. K., Jiang, X., Xu, X., & Yu, J. (2013). Enhanced oral bioavailability and tissue distribution of a new potential anticancer agent, Flammulina velutipes sterols, through liposomal encapsulation. Journal of agricultural and food chemistry, 61(25), 5961–5971. https://doi.org/10.1021/jf3055278
56. Chang, H. H., Hsieh, K. Y., Yeh, C. H., Tu, Y. P., & Sheu, F. (2010). Oral administration of an Enoki mushroom protein FVE activates innate and adaptive immunity and induces anti-tumor activity against murine hepatocellular carcinoma. International immunopharmacology, 10(2), 239–246. https://doi.org/10.1016/j.intimp.2009.10.017
57. Jiang, S. M., Xiao, Z. M., & Xu, Z. H. (1999). Inhibitory activity of polysaccharide extracts from three kinds of edible fungi on proliferation of human hepatoma SMMC-7721 cell and mouse implanted S180 tumor. World journal of gastroenterology, 5(5), 404–407. https://doi.org/10.3748/wjg.v5.i5.404
58. Chang, Y. C., Hsiao, Y. M., Wu, M. F., Ou, C. C., Lin, Y. W., Lue, K. H., & Ko, J. L. (2013). Interruption of lung cancer cell migration and proliferation by fungal immunomodulatory protein FIP-fve from Flammulina velutipes. Journal of agricultural and food chemistry, 61(49), 12044–12052. https://doi.org/10.1021/jf4030272
59. Ou, C.-C., Hsiao, Y.-M., Hou, T.-Y., Wu, M.-F., & Ko, J.-L. (2015). Fungal immunomodulatory proteins alleviate docetaxel-induced adverse effects. Journal of Functional Foods, 19(Part A), 451–463. https://doi.org/10.1016/j.jff.2015.09.042
60. Gu, Y. H., & Leonard, J. (2006). In vitro effects on proliferation, apoptosis and colony inhibition in ER-dependent and ER-independent human breast cancer cells by selected mushroom species. Oncology reports, 15(2), 417–423. https://pubmed.ncbi.nlm.nih.gov/16391863/ 
61. Mustonen, A. M., Määttänen, M., Kärjä, V., Puukka, K., Aho, J., Saarela, S., & Nieminen, P. (2018). Myo- and cardiotoxic effects of the wild winter mushroom ( Flammulina velutipes) on mice. Experimental biology and medicine (Maywood, N.J.), 243(7), 639–644. https://doi.org/10.1177/1535370218762340
62. Otsuji, K., Ohara, K., Nakamura, M., Amazumi, R., Higa, C., Kakazu, K., & Kondo, Y. (2015). Arerugi = [Allergy], 64(1), 63–67. https://pubmed.ncbi.nlm.nih.gov/25779064/ 
63. Shingaki, T., Hiraguchi, Y., Gen, M., Yoshino, S., Kumagai, Y., Ebishima, Y., Owa, K., Otuji, K., Kondou, Y., & Suehiro, Y. (2017). Arerugi = [Allergy], 66(10), 1240–1243. https://doi.org/10.15036/arerugi.66.1240
64. Henao SLD, Urrego SA, Cano AM, Higuita EA. Randomized Clinical Trial for the Evaluation of Immune Modulation by Yogurt Enriched with β-Glucans from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), in Children from Medellin, Colombia. Int J Med Mushrooms. 2018;20(8):705-716. doi: 10.1615/IntJMedMushrooms.2018026986. PMID: 30317947. https://pubmed.ncbi.nlm.nih.gov/30317947/